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Multiplex-PCR for differentiation of Mycobacterium bovis from Mycobacterium tuberculosis complex

Spositto, F.L.E.; Campanerut, P.A.Z.; Ghiraldi, L.D.; Leite, C.Q.F.; Hirata, M.H.; Hirata, R.D.C.; Siqueira, V.L.D.; Cardoso, R. Fressatti.

Braz. j. microbiol ; 45(3): 841-843, July-Sept. 2014. ilus

Article in English | LILACS | ID: lil-727011

ABSTRACT

We evaluated a multiplex-PCR to differentiate Mycobacterium bovis from M. tuberculosis Complex (MTC) by one step amplification based on simultaneous detection of pncA 169C > G change in M. bovis and the IS6110 present in MTC species. Our findings showed the proposed multiplex-PCR is a very useful tool for complementation in differentiating M. bovis from other cultured MTC species.

Subject(s)

Bacteriological Techniques/methods , Molecular Diagnostic Techniques/methods , Multiplex Polymerase Chain Reaction/methods , Mycobacterium bovis/isolation & purification , Mycobacterium tuberculosis/isolation & purification , Tuberculosis/microbiology , Amidohydrolases/genetics , DNA Transposable Elements , DNA, Bacterial/genetics , Mycobacterium bovis/classification , Mycobacterium bovis/genetics , Mycobacterium tuberculosis/classification , Mycobacterium tuberculosis/genetics , Tuberculosis/diagnosis

ABSTRACT

Subject(s)

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